Filling the syringe and de-gassing the buffer oil

It is important to remove all gases from the system as they can form bubbles and cause anomalies in the data. Refill the system with de-gassed oil any time when cleaning it, or if it has had an oil leak for any reason.

Required materials

Buffer fluid
Syringe with a stopper and needle
Syringe kickstand

Recommended handling materials

Latex or nitrile gloves
Lint-free tissues
Protective coat

Buffer oil is not a hazardous substance, but it is recommended to practice good industrial hygiene and safety practices, and to use this material in a well-ventilated space.

Filling the syringe

Step	Description
1	Remove the stopper from the syringe.
2	Install the needle.
3	Draw 1-2ml of the oil into the syringe.

De-gassing the buffer oil

Step	Description	Image
1	Invert the syringe so that the needle is facing up and pull any remaining oil out of the needle into the syringe.
2	Remove the needle.
3	Gently push the plunger to purge the air from the syringe.
4	Install the stopper.
5	Reverse the syringe so that the stopper is facing down.
6	Draw out the plunger of the syringe past the 10ml point.
7	Install the syringe kickstand so that it cups the plunger and supports it in the drawn-out position. The syringe will brace against the flange on the plunger and the barrel.
8	Leave the syringe in the reverse position for about an hour.
9	Remove the kickstand.
10	Invert the syringe so that the tip is facing up.
11	Remove the stopper.
12	Purge any air from the syringe